FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_vglut2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_vglut2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37275036
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG100350.025.6117722
AGTACGG154100.025.1856720
GTACGGG157700.024.63234121
GAGTACG168250.023.252119
AGAGTAC289700.022.233118
CGCAGAG293750.021.90293315
GCAGTGG325100.020.1517262
AGTGGTA332150.019.8281294
ACGCAGA326650.019.69696214
GTGGTAT337550.019.429245
GAGTACT168900.019.09789820
GGTATCA340500.019.0580167
AACGCAG340300.018.90668113
CAGAGTA345750.018.79848917
AGCAGTG345600.018.7684041
GCAGAGT356100.018.2326416
TCAACGC364550.017.8572411
TGGTATC369750.017.643926
GTATCAA385700.016.8961938
ATCAACG402800.016.18729410
Produced by FastQC (version 0.11.2)