FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_vglut1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_vglut1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54470863
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG203000.024.74372120
GTACGGG202200.024.7048321
TACGGGG129500.023.92239622
CGCAGAG386350.022.70723715
AGAGTAC383450.022.69009818
GAGTACG220700.022.665519
ACGCAGA441950.019.83506214
GAGTACT229800.019.18582520
AGTGGTA461150.019.1813964
GCAGTGG463700.019.1353842
GGTATCA467150.019.0013777
CAGAGTA472650.018.6123917
GTGGTAT476500.018.6070485
AACGCAG475200.018.49787513
TCCGATC194150.018.2668196
GCAGAGT485850.018.1208316
AGCAGTG498850.017.7333491
TCAACGC498100.017.66833311
TGGTATC510450.017.3492186
CCGATCT208200.017.0173287
Produced by FastQC (version 0.11.2)