FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_vgat2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_vgat2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33771246
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG135800.025.25135221
TACGGGG90800.025.11390922
AGTACGG137500.025.08995420
AGAGTAC251100.022.91662218
CGCAGAG256600.022.5193715
GAGTACG157350.022.2755419
GCAGTGG288600.020.3096682
ACGCAGA284400.020.23319814
GAGTACT143600.020.20431320
AGTGGTA288350.020.1239744
GTGGTAT295250.019.6889065
CAGAGTA300000.019.25023817
AACGCAG305550.018.97918913
GGTATCA306450.018.9014137
GCAGAGT314700.018.36186416
AGCAGTG313100.018.3362871
TCAACGC317050.018.1386611
TGGTATC329500.017.6005066
GTATCAA334800.017.3421088
ATCAACG352100.016.46053110
Produced by FastQC (version 0.11.2)