FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_vgat1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_vgat1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39558481
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG140900.025.3901321
AGTACGG149200.024.11662120
TACGGGG96300.024.10946522
AGAGTAC278300.021.99073618
CGCAGAG281400.021.8955115
GAGTACG179100.020.36004619
GAGTACT166450.019.9384920
ACGCAGA315400.019.57900814
AGTGGTA328000.019.1005924
GCAGTGG331550.018.8438822
GTGGTAT334450.018.6497785
GGTATCA336200.018.5217787
CAGAGTA338150.018.30265217
GCAGAGT341200.018.19957416
AACGCAG341550.018.11017213
TCAACGC348450.017.81102611
AGCAGTG356550.017.504551
TGGTATC367100.017.1320276
GTATCAA374350.016.6526978
CAACGCA377450.016.46993612
Produced by FastQC (version 0.11.2)