FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_vach2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_vach2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37404819
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG155100.025.15652821
AGTACGG158900.024.59851320
TACGGGG101750.024.51360922
AGAGTAC291900.023.09365518
CGCAGAG300100.022.74988215
GAGTACG174300.022.40532719
AGTGGTA333200.020.6046314
GCAGTGG335150.020.5258392
ACGCAGA338850.020.13811114
GTGGTAT342050.020.071765
GAGTACT166400.020.06895820
GGTATCA342550.019.991497
CAGAGTA345850.019.78054817
GCAGAGT354500.019.4340316
AGCAGTG355750.019.2807641
AACGCAG357500.019.01027313
TCAACGC360750.018.8774911
TGGTATC374500.018.323356
GTATCAA375850.018.1101368
CAACGCA394850.017.34309212
Produced by FastQC (version 0.11.2)