FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_vach1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_vach1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39071589
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470080.12031248588328465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG151750.026.21221
TACGGGG101100.025.62657222
AGTACGG160600.024.96130820
AGAGTAC295050.023.5605918
CGCAGAG295900.023.46938315
GAGTACG187350.021.74717919
GCAGTGG335650.020.9372
AGTGGTA337550.020.839594
ACGCAGA335550.020.68596614
CAGAGTA344550.020.35594717
GTGGTAT344850.020.2584385
TCAACGC346200.020.1490311
AACGCAG353150.019.78172913
GGTATCA358900.019.6380217
GCAGAGT357650.019.6199216
AGCAGTG364200.019.1930051
GAGTACT174050.018.81043820
TGGTATC372400.018.7875216
GTATCAA383600.018.4005578
CAACGCA380500.018.2963712
Produced by FastQC (version 0.11.2)