FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_th2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_th2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27539638
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG64950.025.8144222
AGTACGG95400.024.80720520
GTACGGG96650.024.73622721
CGCAGAG193400.021.7264615
AGAGTAC196600.021.49577318
GAGTACT120500.021.18589220
ACGCAGA214750.019.48617414
GAGTACG124050.019.4393519
AGTGGTA222000.019.1767644
CAGAGTA225400.018.9788517
GCAGTGG227900.018.7557222
GGTATCA233100.018.4263887
TCAACGC229950.018.30339411
GTGGTAT234000.018.2825
AACGCAG232550.018.09855813
AGCAGTG234250.017.6890341
GCAGAGT241050.017.63203816
TGGTATC253050.016.9193366
GTATCAA257250.016.6697438
CAACGCA254150.016.62824812
Produced by FastQC (version 0.11.2)