FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_th1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_th1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53070884
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG195550.024.3633621
TACGGGG132050.023.69582422
AGTACGG201050.023.66270420
CGCAGAG374750.021.4493815
AGAGTAC373600.021.3862718
GAGTACT215400.021.205420
GCAGTGG423350.019.1748092
ACGCAGA422250.019.06932614
GAGTACG256900.019.02869819
AGTGGTA431900.018.7155174
CAGAGTA438900.018.41664317
AGCAGTG439000.018.3590641
TCAACGC441900.018.15100711
GGTATCA448350.018.120847
GTGGTAT450400.017.9545295
AACGCAG450850.017.8365113
GCAGAGT464900.017.41625816
CCGATCT190000.016.7228627
TGGTATC487450.016.6251876
TCCGATC196650.016.3331266
Produced by FastQC (version 0.11.2)