FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_nut2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_nut2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17688376
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG156550.054.0160134
CGTCTGA171750.050.89925417
CTTAGGT165400.050.733236
TTAGGTA172500.048.58532337
CACGTCT182000.048.5064215
TAGGTAT169850.047.77982738
ACGTCTG185850.047.42746416
GTCACCC181350.046.62604530
GCACACG192350.045.89612612
CACACGT193600.045.61760713
ACACGTC194500.045.53093714
AGATCGG200950.045.123371
CCTTAGG187150.044.9838735
GTCTGAA196750.044.3440818
AGAGCAC201300.043.993769
AGGTATC153850.043.91285339
GAGCACA203350.043.6179910
GATCGGA208100.043.534922
TCACCCT195300.043.31339631
AGCACAC204900.043.085511
Produced by FastQC (version 0.11.2)