FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_nut1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_nut1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18264903
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG46850.026.06715822
GTACGGG73350.025.30345221
AGTACGG74100.025.23384720
AGAGTAC143200.025.0066518
GAGTACG76700.024.73822619
CGCAGAG147350.024.37239515
GCAGTGG169000.021.6792242
AGTGGTA168100.021.6515734
ACGCAGA165600.021.5617314
CAGAGTA170100.021.33603917
GGTATCA171200.021.2390797
GTGGTAT173550.021.0115225
GCAGAGT176250.020.80663116
AACGCAG172350.020.69703113
AGCAGTG178150.020.2767771
TCAACGC178800.019.89263511
GAGTACT85200.019.8407319
TGGTATC184050.019.7190976
GTATCAA193150.018.8970748
CAACGCA192200.018.7030812
Produced by FastQC (version 0.11.2)