FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_fasc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_fasc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63383091
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA999650.15771556486571472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG228700.029.053222
GTACGGG342300.028.65308221
AGTACGG346100.028.3784720
GAGTACG360150.027.50119419
AGAGTAC697500.026.19422118
CGCAGAG704050.026.03364615
GCAGTGG738350.025.0257172
AGTGGTA741850.024.847334
GGTATCA755900.024.3760747
AGCAGTG759450.024.1370321
ACGCAGA762250.024.04605114
GTGGTAT766750.024.0270375
CAGAGTA791550.023.2737517
AACGCAG789450.023.26113113
GCAGAGT793850.023.16702716
TCAACGC805350.022.73349411
TGGTATC823550.022.332236
GTATCAA840750.021.9119648
CAACGCA867550.021.22676312
ATCAACG884450.020.84086410
Produced by FastQC (version 0.11.2)