FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_fasc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_fasc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48277523
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG117750.025.95849222
AGTACGG184700.023.79618820
GTACGGG184950.023.59599921
AGAGTAC344850.022.22889118
CGCAGAG351750.021.86120615
GAGTACG207250.021.4233819
GCAGTGG390450.019.9510442
GTGGTAT389500.019.734175
AGTGGTA393400.019.6612474
ACGCAGA393000.019.62818314
GAGTACT200200.019.11051420
AACGCAG415400.018.51982713
CAGAGTA419700.018.39601517
GGTATCA427450.018.231937
GCAGAGT444200.017.42786616
AGCAGTG449150.017.1301441
TCAACGC450800.017.1191611
TGGTATC454700.017.0334116
GTATCAA478200.016.1672388
ATCAACG501050.015.47810710
Produced by FastQC (version 0.11.2)