FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n01_arr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n01_arr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26537722
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG69400.025.20271722
GTACGGG108700.024.50111421
AGTACGG108800.024.4788720
CGCAGAG199800.022.65368515
GAGTACG119100.022.5646119
AGAGTAC200950.022.52412618
GCAGTGG228750.020.088662
ACGCAGA226900.019.88742814
GTGGTAT234450.019.3947285
GGTATCA237050.019.3853557
AGTGGTA235850.019.3670774
GAGTACT118100.019.10428620
CAGAGTA242400.018.92875117
AACGCAG240950.018.88502313
GCAGAGT241850.018.87191216
AGCAGTG242750.018.5195261
TCAACGC253900.018.05795311
TGGTATC258200.017.8779776
GTATCAA267500.017.0885358
CAACGCA280200.016.39969412