FastQCFastQC Report
Wed 20 Feb 2019
HY5VMBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY5VMBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426754997
Sequences flagged as poor quality0
Sequence length98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10537160.24691357041098688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT17578550.072.959451
AGCAGTG20144200.063.4437032
CAGTGGT23253500.054.8128284
AGTGGTA23737500.053.7865835
GCAGTGG24185800.052.795983
GGTATCA26490100.048.149528
GTGGTAT26769250.047.658666
GTATCAA26809450.047.5801479
TGGTATC26915800.047.313347
AGAGTAC26235000.027.62649718-19
CAACGCA26506050.027.28555912-13
GCAGAGT26808100.027.01888816-17
ATCAACG26891700.026.96325110-11
AGTACAT27030000.026.76230620-21
TACATGG27315300.026.34208722-23
ACGCAGA27727650.026.1759814-15
CATGGGG21854650.024.8093924-25
CAGAGTA26191600.024.4976318-19
AACGCAG26496400.024.1927814-15
CGCAGAG26643600.024.10748516-17
Produced by FastQC (version 0.11.5)