FastQCFastQC Report
Wed 20 Feb 2019
HY5VMBGX9_n02_S2_maize10x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY5VMBGX9_n02_S2_maize10x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39695418
Sequences flagged as poor quality0
Sequence length98
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG720650.1815448825856929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3464650.083.21151
AGCAGTG3908050.073.561812
GCAGTGG4766400.060.213363
CAGTGGT4815150.059.526164
AGTGGTA5000800.057.4719965
GTGGTAT5491700.052.2798086
GGTATCA5497550.052.1786468
TGGTATC5496950.052.125357
GTATCAA5577100.051.467539
AGAGTAC5549850.029.12623418-19
CAACGCA5544400.029.10847312-13
ATCAACG5583150.028.95983310-11
GCAGAGT5579700.028.9476716-17
AGTACAT5607650.028.7338320-21
ACGCAGA5634200.028.71712314-15
TACATGG5608400.028.55715222-23
CATGGGG4390700.027.47952724-25
AACGCAG5533450.026.0531814-15
CAGAGTA5546900.026.00821518-19
CGCAGAG5563700.025.93871316-17
Produced by FastQC (version 0.11.5)