FastQCFastQC Report
Wed 20 Feb 2019
HY5VMBGX9_n02_S1_maize10x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY5VMBGX9_n02_S1_maize10x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94066409
Sequences flagged as poor quality0
Sequence length98
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1867900.19857247872617315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2115250.057.5347181
AGTGGTA2513800.048.192235
CAGTGGT2571550.047.063794
AGCAGTG2650300.045.7414862
GCAGTGG2978000.040.8247763
GGTATCA2964700.040.779528
TGGTATC3015500.040.0414477
GTGGTAT3033350.039.925126
GTATCAA3052250.039.466989
AGAGTAC2883100.024.48181218-19
CAACGCA2903150.024.16644512-13
GCAGAGT2973800.023.78314816-17
ACGCAGA3035500.023.25480714-15
ATCAACG3077200.022.95226310-11
AGTACAT3102750.022.71469320-21
TACATGG3204350.022.00580822-23
AACGCAG2813850.021.53539314-15
CAGAGTA2872150.021.21052218-19
TATCAAC2906200.020.82415810-11
CGCAGAG3017250.020.29729316-17
Produced by FastQC (version 0.11.5)