FastQCFastQC Report
Wed 20 Feb 2019
HY5VMBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY5VMBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426754997
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA1928600.018.3288081
GGGAGAT1374500.018.1522391
GCGGACA1310850.017.8701113
TAGCCGG1792850.017.5872041
CACGGTA2102050.017.5807278
CTGATCC2723200.017.4818121
CGGGCGT850050.017.4620973
CGGACAT1309850.017.4418544
CGATTGA1660300.017.4384481
CGGCGGT1062950.017.4311719
CAGCCGA1400050.017.3879361
CGGAGCT1040100.017.3426931
TCACGTA1808600.017.34215710
GTGGGAG927250.017.283592
CTAAGAC1147750.017.2781051
TGCACCT1903950.017.2477261
CCGGGAT973250.017.2438411
TGCGGAC1362750.017.1930082
CAATACG1511500.017.1886838
CCGTACT1499750.017.1759411
Produced by FastQC (version 0.11.5)