FastQCFastQC Report
Wed 20 Feb 2019
HY5VMBGX9_n01_S2_maize10x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY5VMBGX9_n01_S2_maize10x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39695418
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTCTGCATCGTTTATGGTTGAGA555930.1400489094232488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG238900.018.7750916
GGAGGGA124800.018.6393576
GGGAGAT165250.018.6364941
CGATGGC273250.018.4011361
AAGGCGT235800.018.3883347
CAATGGA212250.018.3367398
GAGGAGG133850.018.3127274
GCTAAGG247300.018.2987984
CGGAGCT177400.018.2847611
GATGGCT242600.018.2370512
CTAAGGC238400.018.231235
GGCGTAC249950.018.2071919
AAGCTAG175100.018.20097
ATGGCTC243100.018.170543
ACGAGGA144450.018.1486951
GGGACCT119450.018.095251
ACCCACT251600.018.060431
ATGCGAT139250.018.007571
AGGAGGG129150.018.003675
GGATGCG117850.017.980049
Produced by FastQC (version 0.11.5)