FastQCFastQC Report
Wed 20 Feb 2019
HY5VMBGX9_n01_S1_maize10x.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY5VMBGX9_n01_S1_maize10x.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94066409
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA579600.019.0424181
GCGGACA417450.018.9806423
TGCGGAC419000.018.931962
CAGATGG739500.018.7491598
GCGAGAA413750.018.7369061
GCAGATG413600.018.5855857
TAGCCGG551050.018.3482781
CAAGAAA570900.018.3322531
ACATACG582450.018.3138791
CAACCTC664000.018.2999781
CGGACAT447550.018.2069824
CGGGCGT277650.018.1138063
TCACGTA603500.018.0643210
CGTGCTC442550.018.0487483
GTGGGAG315600.018.0334362
CGATTGA485850.018.004681
CTGATCC732800.017.990411
CCGGTTG447500.017.9857855
TGTAGCG393750.017.8920577
CCCAATC582350.017.88251
Produced by FastQC (version 0.11.5)