FastQCFastQC Report
Tue 18 May 2021
HY2CLDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY2CLDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966930910
Sequences flagged as poor quality0
Sequence length10
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAAGATA51576954026.22204660965951No Hit
ATCAGTCTAA47678885024.240243903635637No Hit
CAGGGTTGGC46606164623.69486613030043No Hit
AGGTAACACT37817508219.22665814428632No Hit
AGATCTCGGT575816392.9274866090746423Illumina Single End PCR Primer 1 (100% over 10bp)
AAAGGTAGTA67397920.3426552486279246No Hit
ATAGATGCTC64404490.327436462930973No Hit
GGGGGGGGGG61690680.31363928283581655No Hit
CTCCAAGTTC61047670.31037017970295666No Hit
GAAGTTAGGG47917630.24361623357680623No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)