FastQCFastQC Report
Tue 18 May 2021
HY2CLDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY2CLDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966930910
Sequences flagged as poor quality0
Sequence length10
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGCGAG51320514326.09167105925444No Hit
CACTACGAAA47620415624.21051769428953No Hit
GTGGATCAAA45438889623.101416205818843No Hit
GTAACATGCG37306825618.967023910361956No Hit
GGGGGGGGGG671346733.4131688438410883No Hit
GCCCAAGGGT68201860.34674252996512217No Hit
GTAGCCCTGT62977550.32018181055479983No Hit
CGAAGTATAC59944300.3047605774826122No Hit
GAACGCGTGA50516570.2568294073938774No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)