FastQCFastQC Report
Tue 29 Mar 2022
HY2C2DRXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY2C2DRXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896493449
Sequences flagged as poor quality0
Sequence length10
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGTTGGC35649796739.765819526919934No Hit
AGGTAACACT24835478727.702911524565977No Hit
GATGAAGATA12934239114.427589085483659No Hit
ATCAGTCTAA11833638513.199916310821811No Hit
AGATCTCGGT161103881.7970446987616528Illumina Single End PCR Primer 1 (100% over 10bp)
AGGTACCACT20676670.2306393875277498No Hit
GGGGGGGGGG18592490.2073912533408819No Hit
NAGGGTTGGC14586070.16270135622597282No Hit
AAGTAAAACA14003850.15620694178658745No Hit
NGGTAACACT10144310.11315542808835405No Hit
CGGTAACACT9889300.11031090088869126No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)