FastQCFastQC Report
Sat 23 Nov 2019
HWWJ3AFXY_n01_CSA_sgRNA_10plates.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWWJ3AFXY_n01_CSA_sgRNA_10plates.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79432103
Sequences flagged as poor quality0
Sequence length162
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15624691.9670497707960723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCC13269950.0152.16861
TTCGCCG1408650.0150.70587
GTACGAC425900.0148.548231
CTTCGCC314000.0143.762336
TTACGCC431600.0143.633886
ATTCGCC371000.0143.302676
TTGCGCC377550.0142.819956
TCCGCCG1043100.0142.526057
GTTCGCC315800.0141.856456
TTTCGCC491400.0141.705756
CTCGCCG1011100.0141.423667
GGCGCCG1042200.0141.28777
TCGCCGT4659800.0140.965678
GCGCCGT4332700.0139.646018
CTACGCC279650.0139.58776
AGGCGCC251250.0138.88596
TTCCGCC331300.0138.703116
TCACGCC299500.0137.730476
CGTCGCC215750.0137.52296
TACGACG517350.0136.662462
Produced by FastQC (version 0.11.5)