FastQCFastQC Report
Fri 1 Mar 2024
HWW5MDRX3_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWW5MDRX3_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907118255
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG236329731.2391980905242816Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA93247320.4889435657989651Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCCGTCCGAATTGTAGTC76625120.4017848384551277Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCGGCGACGCATCATTCA53247000.27920135450646194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCACGACTCTCGGCAACGGAT48410760.25384246557904194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACACGACTCTCGGCAACGG46174200.24211503339629037Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGGCGAGAGCGGGTCGCC43770550.22951146257052632Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42034540.22040867098721156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGCGACGCATCATTCAA39167290.20537420737970963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGTGCCGGCGACGCATCAT36219720.18991858478120435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGCCGGCGACGCATCATT36063730.18910064913620156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGCTCTGGATACATTAGCA34466850.18072738756307485Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCGTTCACGCCCGGTCGTA33404240.1751555778590143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA32219730.16894458387951408Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACACGACTCTCGGCAACG30327950.15902501022413001Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCGTCCGAATTGTAGTCT24181190.1267943922019665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACGCATCATTCAAATTT23588680.12368755811631618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGA22118080.11597644740703299Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCGGGCCGATCCGGGCGG20661140.10833696308989503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGGCGCCGTCAACGAGTC20538050.10769153903358762Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGGGACGTAGTCAACGCG19573240.10263254493361242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCAAGTGAAATACCAC19284070.10111627818276009Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)