FastQCFastQC Report
Fri 1 Mar 2024
HWW5MDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWW5MDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907118255
Sequences flagged as poor quality0
Sequence length10
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGTTAGGG39641776020.78621810476037No Hit
TCCTCGAATC37489296919.657562818515416No Hit
CTCCAAGTTC35489989118.609223107667226No Hit
GTAACAGGAA30837276516.169567051834445No Hit
AAAGGTAGTA22834904811.973512780412246No Hit
TTTAGACCAT1439890117.550082991576209No Hit
AGATCTCGGT390511722.047653410983683Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG64288700.3370986556887633No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)