FastQCFastQC Report
Sat 1 Apr 2023
HWW3NDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWW3NDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944157065
Sequences flagged as poor quality0
Sequence length10
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGTTGGC26146090527.692522218218002No Hit
ATCAGTCTAA25701881727.2220403286396No Hit
GATGAAGATA21096993222.34479196530717No Hit
AGGTAACACT16196742817.1547122829611No Hit
AGATCTCGGT188862092.0003249141603363Illumina Single End PCR Primer 1 (100% over 10bp)
ATCAGTCTAG47963040.5079985288252861No Hit
GGGGGGGGGG25597090.2711105063859264No Hit
GATGACGATA16838160.17834066623226508No Hit
CAGGGGTGGC13206340.13987439685154504No Hit
AGGTACCACT10592940.11219468023575081No Hit
AGTTCTCGGT9641820.10212093260139933No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)