FastQCFastQC Report
Sat 7 Dec 2019
HWVLTAFXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVLTAFXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202573250
Sequences flagged as poor quality0
Sequence length91
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7704310.3803221797547307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5879450.069.01741
AGCAGTG6119050.066.120172
GCAGTGG6732900.060.048943
CAGTGGT6961450.057.9285054
AGTGGTA7460450.054.163135
TGGTATC7675350.052.461257
GTGGTAT7768100.051.9470526
GGTATCA7828200.051.4621478
GTATCAA8248400.048.6861469
CATGGGG4587050.029.67577624-25
CAACGCA7726850.029.03850412-13
AGAGTAC7760650.028.83295618-19
GCAGAGT7828550.028.70194816-17
TACATGG7729000.028.44690922-23
ATCAACG8037850.027.93648310-11
ACGCAGA8177750.027.52032514-15
ACATGGG7420750.026.56986424-25
CAGAGTA7776100.026.00728418-19
AGTACAT8638800.025.78626420-21
CATGGGA2428800.025.69593624-25
Produced by FastQC (version 0.11.5)