FastQCFastQC Report
Sat 7 Dec 2019
HWVLTAFXY_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVLTAFXY_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202573250
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG198000.019.2780274
TACCGGG621550.019.0901051
ACCGGGG326500.018.9922622
GACCGTG391850.018.7888551
CCGGGGT331150.018.621953
GGGGTGC182950.018.4951465
GGGGTGG130100.018.3478975
TGGGGTG232150.018.2522264
TGTGAAG551050.018.237363
GTCGGAC285550.018.170647
TGAAGGG386400.018.0121085
GGGCTAC442000.017.9364381
GAAGGGC226600.017.806766
TGACGAG377050.017.6645710
GCTAGCG302050.017.46578810
GGATCTA684200.017.4417761
AAATGGA448000.017.4113481
GTGAAGG605150.017.3195634
GTGCGTG290600.017.31291
CTTGGGG246350.017.2899882