FastQCFastQC Report
Mon 27 Nov 2023
HWVLJBGXT_n01_HW19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVLJBGXT_n01_HW19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60121365
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1628120.27080556138404377TruSeq Adapter, Index 6 (100% over 50bp)
CTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGAAA1396900.23234668740471875No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA1177330.19582556051413672No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA1053000.1751457239868057No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT1022260.17003273295607976No Hit
GTCGTATGCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGC928260.1543976920683687No Hit
CCCTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGA844290.14043094330942088No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC841430.13995523887390116No Hit
CTGGAAACTTACACCTATACGCCACATTTCGAAGCAGCCAAAGACTGATC782240.1301101530213095No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG775410.1289741176036173No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA754860.1255560315372081No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG713270.11863835759550702No Hit
CCGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATA692600.11520031190243268No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG687540.11435868097805166No Hit
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG668230.11114684438718249No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA635480.10569952960981507No Hit
GCGTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCG634000.10545336088094474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)