FastQCFastQC Report
Mon 27 Nov 2023
HWVLJBGXT_n01_HW14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVLJBGXT_n01_HW14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49355458
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1117440.22640657088016486TruSeq Adapter, Index 1 (100% over 50bp)
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA706430.14313107984936538No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG615880.12478457802985031No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA577710.11705088422034296No Hit
CTGGAAACTTACACCTATACGCCACATTTCGAAGCAGCCAAAGACTGATC559320.11332485254214436No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG543240.11006685420688427No Hit
GGCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGG541310.10967581336191837No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT541170.10964744770477057No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC502850.10188336211974774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)