FastQCFastQC Report
Mon 27 Nov 2023
HWVLJBGXT_n01_DR02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVLJBGXT_n01_DR02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28640017
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4745901.657087005220702TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC3025271.0563087305430021TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGG793450.2770424333197847TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATTC656280.22914790867617155TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTGC622450.2173357648495809TruSeq Adapter, Index 12 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATGC600940.20982529444727635TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGG593930.2073776702018019TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATTC587920.20527920776024677TruSeq Adapter, Index 12 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC541640.18911999947486066TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTGG507440.1771786657808199TruSeq Adapter, Index 12 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGC488640.17061442386713666TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGTTGG399410.13945871610341573TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGGATGC334520.11680160664709102TruSeq Adapter, Index 12 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTTTGG329870.11517800425886618TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATTG326320.1139384798549526TruSeq Adapter, Index 12 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATGG323220.11285607826280271TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATGC309380.10802367889655932TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTTG307460.10735328823303421TruSeq Adapter, Index 12 (97% over 46bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)