FastQCFastQC Report
Mon 27 Nov 2023
HWVLJBGXT_n01_DR01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVLJBGXT_n01_DR01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25560996
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC22086208.640586618768689TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATTC1094200.4280740860019696TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGG984580.3851884331893796TruSeq Adapter, Index 11 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTTGC975370.38158528720868307TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAGCTCGTATGC943720.3692031405974947TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTATGC841290.3291303672204322TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGC763950.29887333028806856TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACCTCTCGTATGC436220.1706584516503191TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTTGG324280.12686516597397066TruSeq Adapter, Index 11 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCGTATGC284990.11149409044937059TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAAGC283050.11073512158915873TruSeq Adapter, Index 11 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)