FastQCFastQC Report
Thu 30 Nov 2023
HWVKNBGXT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVKNBGXT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293901683
Sequences flagged as poor quality0
Sequence length6
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAA64705812.201614136384513No Hit
TAAAAA30269201.0299090393436092No Hit
AAAAAT22317260.7593444097426281No Hit
AAAATA17231730.586309333927836No Hit
GAAAAA16942640.5764730513639148No Hit
CAAAAA16349790.5563013397238694No Hit
ATAAAA16311360.5549937595968105No Hit
AATAAA16295780.5544636503493585No Hit
AAATAA15857580.5395539024524743No Hit
TAAAAT13103570.4458487568443084No Hit
TTAAAA10630660.3617080341795797No Hit
ACAAAA10344840.3519830133126526No Hit
AAAAAC10311250.35084011410713833No Hit
TAAATA9711330.33042784583169604No Hit
TATAAA9231070.31408700711659415No Hit
TAATAA8985760.3057403383430098No Hit
AAATTA8429180.28680271286503656No Hit
AAATAT8391420.285517929477117No Hit
AAAATT8361720.2845073874585468No Hit
AATAAT8123550.27640365706922476No Hit
AAAACA7870700.26780043991786195No Hit
ATAAAT7588030.25818259774987407No Hit
AATATA7454060.2536242706714953No Hit
AAAAAG7410260.25213397638148266No Hit
AACAAA7309330.24869983476753346No Hit
AATTAA7258190.24695979709650046No Hit
GAAAAT7007340.23842462991271815No Hit
AAACAA6875760.23394762254559803No Hit
CAAAAT6313760.2148255816554817No Hit
GTAAAA6296640.21424307393299274No Hit
ATTAAA6227160.2118790180592467No Hit
ATAATA6165140.20976878856457584No Hit
TTAAAT6089790.20720500603598108No Hit
TCAAAA6042380.20559188155448568No Hit
AATTTA6002430.20423258345206552No Hit
TAATAT5779960.19666304530825024No Hit
TAAATT5713510.19440208513538865No Hit
TAAAAC5696690.19382978490803676No Hit
AAATTT5659700.19257120075763567No Hit
TATAAT5637900.19182945611100838No Hit
ATATAA5547470.18875257682685675No Hit
TAATTA5384360.18320276172083028No Hit
TTTTTT5343790.18182236812846014No Hit
GAAATA5262590.17905953944469247No Hit
CTAAAA5182300.1763276734961739No Hit
AATTTT5114260.1740126135990858No Hit
AATTAT5006870.17035867058985166No Hit
CAAATA4937270.16799053171805076No Hit
GATAAA4909810.1670562056631707No Hit
TATATA4858490.16531004349505546No Hit
AATATT4856240.16523348728152742No Hit
TATTTT4842460.16476462300489786No Hit
GAATAA4818910.16396333463663765No Hit
TTTAAA4775670.16249209433754755No Hit
TATTAA4759440.1619398688506319No Hit
CATAAA4644270.1580212114675097No Hit
CAATAA4538410.15441932668347463No Hit
TTAATA4511220.15349418737421794No Hit
TAAAAG4491790.1528330819391735No Hit
AGAAAA4488260.15271297374639398No Hit
TAATTT4391520.1494213968145259No Hit
TATTTA4377140.1489321175476222No Hit
ACAAAT4291300.14601141293906778No Hit
TAAACA4247370.14451669540116244No Hit
TTATAA4210840.1432737627433049No Hit
GGGGGG4165300.14172426498149723No Hit
TATTAT4118090.14011794549675988No Hit
ATTTTT4086890.13905636600250432No Hit
ATTAAT3947080.1342993330187905No Hit
GCAAAA3930030.13371920704516688No Hit
TACAAA3915930.13323945477372445No Hit
TATATT3888490.1323058092185202No Hit
CCAAAA3882370.13209757631772392No Hit
AAGAAA3769820.12826806439213212No Hit
ATAATT3762460.1280176405114359No Hit
ATATTA3740360.12726568836967156No Hit
ATTATA3720570.12659233394046268No Hit
TAACAA3711030.12626773559510376No Hit
ATATAT3683560.1253330692903858No Hit
TTTTTA3613110.1229360091823632No Hit
TTTAAT3592790.12224462151174548No Hit
GTAAAT3580640.12183121795869405No Hit
AAAAGA3577580.12172710150829588No Hit
AAAATC3553800.12091798739376391No Hit
ATTTTA3527870.12003571956408293No Hit
AAATAC3514360.11957604203307676No Hit
ATTTAA3491360.11879346740590119No Hit
AATAAC3462460.11781014537436316No Hit
AAAACT3449250.11736067533849406No Hit
ACTAAA3435910.11690678205473223No Hit
ACAATA3409540.11600954323218353No Hit
TTAATT3394310.11549134272905814No Hit
TGAAAA3347190.1138880854928619No Hit
ATAAAC3345750.11383908951620395No Hit
TTATTT3337500.11355838339993446No Hit
TTATAT3333020.11340595147255417No Hit
TTTTAT3326070.11316947783521199No Hit
CAAAAC3297220.1121878570528635No Hit
GAAAAC3246580.11046483187372562No Hit
AACAAT3242660.11033145393726787No Hit
AAACAT3223160.10966796675335813No Hit
TTTATT3212840.10931682892064282No Hit
TTTATA3206750.10910961676936025No Hit
TTATTA3206060.10908613953054498No Hit
AAATCA3194620.10869689371598461No Hit
ATATTT3177410.10811132374495454No Hit
ACATAA3100890.10550773198532518No Hit
TTTTAA3097140.10538013829611177No Hit
TCAAAT3093670.10526207160235963No Hit
ATTTAT3084570.10495244424986842No Hit
AAATAG3046190.1036465653719989No Hit
AAACTA3031500.10314673836012023No Hit
AAAGAA3024810.10291911121856352No Hit
ATTATT3023820.10288542648461119No Hit
GTTTTT3013340.10252884465448944No Hit
GAATAT3010680.1024383381976074No Hit
GAAATT2970540.10107257534826707No Hit
AATACA2947350.10028353597417133No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)