FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_tga1_30_n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_tga1_30_n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16502652
Sequences flagged as poor quality0
Sequence length75
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG378547422.938579811293362No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5825393.5299720311620217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5619243.4050527151635994No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3938222.3864164377943617TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA485010.29389821708656283TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAA326490.19784092883980103TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG250100.15155139913269697TruSeq Adapter, Index 15 (97% over 40bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA187080.11336359755995581TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCGTCTGCTTGAAAAAAAAAA171710.10404994300310035TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTGTGCTTGAAAAAAAAAA166800.10107466363588108TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1201250.066.3317715
CGTCTGA1201800.066.2380616
ACACGTC1206350.066.2351613
GCACACG1207550.066.1848311
CACACGT1207400.066.1638812
CACGTCT1205600.066.1613114
CAGTCAC1188350.066.0520727
CTCCAGT1189900.065.9043524
CCAGTCA1189650.065.849226
ATGTCAG1161300.065.8051934
TCCAGTC1189850.065.8031325
TCTGAAC1208200.065.7988718
ACTCCAG1193400.065.795123
GTCACAT1170750.065.77772529
GTCTGAA1210450.065.7476117
TGTCAGA1157600.065.7264435
ACATGTC1170550.065.6895532
CTGAACT1209750.065.6430719
AGCACAC1218900.065.6342410
GAACTCC1202200.065.5937121
Produced by FastQC (version 0.11.2)