FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_tga1_30_n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_tga1_30_n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30995543
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG375650.12119484404580362TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG139150.057.8425946
CGTATGC140650.057.0299444
TCGTATG141800.055.49654443
ATGCCGT144650.055.11800447
GCCGTCT143500.052.72325549
TGCCGTC148850.052.40450748
TCTCGTA83650.051.4717641
GTCGTAT68300.051.21977242
CTCGTAT85450.051.07387542
CCGTCTT150550.050.13973250
TGTCGTA69450.050.12325341
CGTCTGA168150.049.79385416
ACACGTC176450.047.60825713
GATCGGA168200.047.244741
ACGTCTG177450.047.2423815
ATCGGAA179900.047.123982
TCGGAAG182300.046.523123
ATCTCGT92150.046.4243740
GTATGCC174150.046.2771145
ATGTCGT76200.045.63785640
Produced by FastQC (version 0.11.2)