FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_tga1_30_k_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_tga1_30_k_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14146526
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9692386.851420624399234No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4217072.9809933548349608TruSeq Adapter, Index 16 (97% over 40bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3969472.805968051802966No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1352380.9559802880226567TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1195260.8449141506543727TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG618900.43749256884693816TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT164450.1162476215008547TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1156500.066.661446
TATGCCG1152000.066.5502148
GTCCCGA1107950.066.54310636
CCCGTCC1167000.066.5255233
CGTCCCG1165050.066.5026835
GTATGCC1154800.066.46030447
CCGTCCC1166950.066.40964534
CACCCGT1167800.066.3910931
GCCGTCT1118250.066.3704451
TCGTATG1128250.066.3678545
TCACCCG1170100.066.3517530
ATGCCGT1145950.066.3137949
CTCGTAT814500.066.21377644
ACCCGTC1169550.066.2128132
AGTCACC1180200.066.09939628
TCCCGAT1075200.066.0380837
AGGGGGG1931300.066.035381
ACGTCTG1204650.065.9797615
GTCACCC1177950.065.96815529
CAGTCAC1184300.065.92544627
Produced by FastQC (version 0.11.2)