FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_tga1_30_k_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_tga1_30_k_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39941046
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG10842142.714535818616268TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG2888730.7232484597423914TruSeq Adapter, Index 6 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2468880.6181310324221354No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG514720.12886993495363142TruSeq Adapter, Index 6 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1944150.065.9900244
GTATGCC1946100.065.8050445
AGTCACG1989850.065.72848528
TATGCCG1951600.065.6302846
TCGTATG1959800.065.4361643
CTCGTAT1821000.065.2651642
TATCTCG1836450.064.9465939
TCTCGTA1840750.064.56653641
GCCGTCT1941000.064.5113349
ACGTCTG2045900.064.5081715
CAGTCAC2034300.064.34132427
CGTCTGA2053000.064.3063616
GTCACGC2028000.064.3015829
CACACGT2065550.064.127312
CACGTCT2062550.064.0544114
ACGCCAA2031950.064.0345332
ACACGTC2068100.064.01128413
ATGCCGT1989350.063.99979847
CGCCAAT2030600.063.97136333
GCACACG2072900.063.89484411
Produced by FastQC (version 0.11.2)