FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_target_ev_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_target_ev_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32041337
Sequences flagged as poor quality0
Sequence length75
%GC78

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG852367026.602104650002588No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12923774.0334677669661545No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11782513.6772841283121234No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6037521.8842909083350672TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1397910.43628329242315944TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG1396900.43596807461561293TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG556650.17372870551562813TruSeq Adapter, Index 4 (98% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG382430.11935519419804487No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG366210.11429298346695084TruSeq Adapter, Index 4 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1312750.066.6119144
GTATGCC1311550.066.4805245
TCTCGTA966450.066.4760741
AGTCACT1335000.066.4614228
ACTGACC1313150.066.4594332
ACGTCTG1351500.066.4321315
TATGCCG1313400.066.3554646
CGTCTGA1354150.066.3121116
CACACGT1358700.066.2610912
AATCTCG965150.066.2578939
TCTGAAC1354300.066.2459718
ACACGTC1360350.066.1880313
ACCAATC964000.066.0398936
CTGAACT1356850.066.0247519
CCGTCTT1289150.066.0057650
GCCGTCT1293900.065.9979949
GCACACG1367450.065.8295411
CTGACCA1323850.065.82001533
ATGCCGT1317400.065.80847
ATCTCGT971100.065.7990440
Produced by FastQC (version 0.11.2)