FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_target_bzip1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_target_bzip1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37057476
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG5137101.3862519940645714TruSeq Adapter, Index 2 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3740751.0094454355175186No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG1059980.2860367500474128TruSeq Adapter, Index 2 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG801990.21641786936595467No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG554180.14954607270068798TruSeq Adapter, Index 2 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1032700.066.1038144
TCTCGTA896100.065.4996141
GTATGCC1053800.065.34669545
TATGCCG1061700.064.8182246
GATGTAT1106650.064.6909635
CCGTCTT998500.063.9490250
AGTCACC1138200.063.83343528
CCGATGT1133850.063.79935533
GCCGTCT1003250.063.7081549
ATCTCGT936050.063.28997840
ACCGATG1151050.063.0112832
ATGCCGT1080300.062.9136647
TCTGAAC1172000.062.68037818
CTTGAAA1031200.062.5770860
CGTCTGA1176650.062.45948416
GCTTGAA1032750.062.4084859
ACGTCTG1180600.062.2068515
TGCTTGA1013400.061.99675458
CACACGT1187700.061.9811312
ACACGTC1188100.061.92807413
Produced by FastQC (version 0.11.2)