FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_input_30_n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_input_30_n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60356543
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG146900.036.35523648
ATGCCGT145400.036.1608649
TGCCGTC144950.035.96391750
GCACACG162050.035.2341911
CGTATGC156650.034.09237746
GTATGCC161700.032.9421747
GCCGTCT160050.032.39829651
ACGTCTG181250.031.71025315
CACACGT199450.028.9731512
ACACGTC198850.028.92160613
GTCACAG202950.027.60616929
CACGTCT211750.027.20803814
CGTCTGA216700.026.5068916
CAGTCAC217550.026.1497727
CACAGTC219700.025.45442231
TCGTATG215550.025.0645445
AGGGGGG172100.024.4599231
CCAGTCA238350.023.92568426
TCCAGTC245550.023.13970625
CTCGTAT197550.022.79038444
Produced by FastQC (version 0.11.2)