FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_input_30_k_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_input_30_k_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55126480
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC111350.035.66195750
ATGCCGT117000.034.85379449
TATGCCG119400.034.5579348
GCACACG132150.033.23341811
CGTATGC130400.031.6427146
GTATGCC132200.031.62936247
GCCGTCT126500.030.05450451
ACGTCTG154650.028.3083415
CACACGT165200.026.8978312
GTCACAG166200.025.86402329
ACACGTC173450.025.45917313
CCGTATC155550.024.26394838
CACGTCT184400.023.87209514
CGTCTGA190450.023.16815816
CAGTCAC190550.023.08393727
GTTCCGT186650.022.75287835
TCGTATG185700.022.14544545
AGTTCCG207400.020.67615734
TTCCGTA208700.020.54753336
CGTATCT183150.019.96692339
Produced by FastQC (version 0.11.2)