FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_bzip1_30_n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_bzip1_30_n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43920403
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG104150.058.0347132
CGTCCGC105950.056.78799433
TATGCCG106400.055.64044648
GTCCGCA111500.053.7138634
CGTATGC112650.052.58459546
TCCGCAC114300.052.3981535
CTCGTAT103250.051.15717344
TGCCGTC118750.048.77884750
TCTCGTA107150.048.71505443
CGCACAT117900.048.66211337
GCCGTCT119300.047.6286951
ATGCCGT123850.047.5776949
TCGTATG124300.047.48968545
GTCACGT130550.046.7211929
CCGCACA125250.046.68812636
ACACGTC133200.046.4913113
AGTCACG132150.046.28594228
CGTCTGA134550.046.0239516
TCACGTC136550.044.7439630
GATCGGA137200.044.4387361
Produced by FastQC (version 0.11.2)