FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_bzip1_30_n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_bzip1_30_n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60513699
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1034270.1709150187629416TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG312150.053.34967846
CGTATGC314400.052.66089244
GTATGCC321700.051.80887245
GCACACG360250.051.50308211
ATGCCGT323100.050.5165247
TGCCGTC318900.049.61321348
ACGTCTG375350.049.4205815
AGGGGGG325800.049.35351
GCCGTCT308800.048.61033249
ACACGTC398150.046.55662513
CACACGT401950.046.16847612
CACGTCT405750.045.74345814
CGTCTGA406850.045.56016516
AGTCACC411950.044.5444928
CAGTCAC415750.044.38625327
TCGTATG367200.044.28032743
CCAGTCA425800.043.1280726
TCCAGTC431350.042.67674625
AGCACAC447250.041.762610
GAGCACA456150.040.8949249
Produced by FastQC (version 0.11.2)