FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_bzip1_30_k_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_bzip1_30_k_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18857192
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA654040.34683849005726836TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG409000.2168933741566613No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG343770.1823017976377395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG358300.060.832671
TATGCCG158000.059.414548
CGTATGC159400.059.32569546
ATGCCGT159850.058.3599749
GTATGCC162450.057.8929947
GCCGTCT160450.057.60432451
TGCCGTC161500.057.5502550
GCACACG169500.057.29564311
AGTCACG168650.056.5199428
GTCACGT169550.056.05743829
ACGTCTG173600.055.8416215
CACGTGA177650.053.57945331
CGTCTGA181950.053.2979116
ACACGTC182550.053.29366313
TCACGTG178150.053.29337730
TCGTATG175100.053.27721845
CACGTCT184050.052.76448414
CACACGT184400.052.6657312
CAGTCAC185050.051.99587627
CCAGTCA190200.050.64254426
Produced by FastQC (version 0.11.2)