FastQCFastQC Report
Tue 25 Jul 2017
HWVGNBGX2_n01_bzip1_30_k_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVGNBGX2_n01_bzip1_30_k_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30233331
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG612850.2027067411129789TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG176350.055.4612846
GTATGCC179650.054.63463645
CGTATGC179850.054.51633544
ATGCCGT181500.053.77363247
TGCCGTC180700.053.43910648
GCCGTCT180900.052.6936449
GCACACG192500.052.5469711
ACGTCTG195700.051.4398415
GTCACCT202200.049.10416829
CACACGT211900.048.12690712
ACACGTC210250.048.06131413
CGTCTGA211450.047.60830316
CACGTCT216600.046.5879914
TCGTATG210800.046.5444343
AGTCACC214000.046.54133628
CAGTCAC214550.046.53450827
CCAGTCA224100.044.53605326
TCCAGTC227700.043.92275625
AGCACAC231950.043.80351610
CCGTCTT223700.042.82780550
Produced by FastQC (version 0.11.2)