FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23345720
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260706011.167186105204722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA42900.032.5277181
AGCGTCG41800.029.59469610
TCGGAAG63700.024.0792563
ATCGGAA72750.020.5616592
TCGTGTA46650.020.33238614
CGGAAGA84900.018.6000984
TGTAGGG55600.018.33085317
GTGTAGG56750.017.77416416
GTAGGGA59550.015.87981818
GAGCGTC83700.015.4901249
AGTGTAG57500.015.01656328
CGCCGTA19250.014.91962246
GAGTGTA57250.014.77689527
CGTGTAG67300.014.77294915
CGTCGTG91150.013.53356912
GCCGTAT30100.013.49804647
GTGTAGA64950.013.13264329
AGAGCGT103500.012.9612398
AAGAGCG118550.012.6423067
GTCGTGT83850.012.64011913
Produced by FastQC (version 0.11.5)