FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_WT-70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_WT-70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9263464
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5683706.135609745987031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG435650.011.2043121
AGGGGCG31800.06.7216291
AGGGGCC16459.276846E-116.17737441
AGGGGGT40950.06.0754191
AAAGGGG126650.05.776437370
AAAAAGG251400.05.59731570
GCGTCGT10302.700307E-45.436385611
GGGGAAA227200.05.40723959
AAAAGGG169250.05.17047370
CACACCC21703.5106495E-95.00077534
Produced by FastQC (version 0.11.5)