FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_WT-60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_WT-60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6772777
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217206032.07044909348115No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG179840.26553362084710597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG150000.22147488393608705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG144880.2139152078977353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG140460.20738908131775194No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG116630.1722041047564389No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG115090.16993029594802841No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG100690.14866870709016405No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG88690.13095071637527708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG86760.12810107286863276No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG71980.10627841430479697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG70760.10447708524878346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG389850.024.01694970
AAGGGGG354400.022.12309570
GGTCGCC7600.021.18348144
AGCGTCG10500.020.33128411
AAAAGGG522300.020.29882270
AGATCGG10550.019.9288121
GAGCGTC10850.019.67543410
CGTGTAG9450.019.62736116
TGGTCGC8400.018.74907743
CGCCGTA5450.018.6222547
GATCGGA10900.018.3113172
CGTCGTG12000.018.08164413
GTCGTGT11500.017.95484414
GCGTCGT12100.017.93207612
GATCTCG8250.016.54525235
AGAGCGT13600.015.9547449
CTCGGTG8200.015.79272438
AAAAAGG687650.015.72300769
TCGCCGT8700.015.68795746
GTCGCCG10800.014.90645545
Produced by FastQC (version 0.11.5)