FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_WT-50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_WT-50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11580571
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170903614.757786986496606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG242050.015.98023770
AAAAGGG314700.014.39340770
AAGGGGG263300.012.3506870
GGTCGCC9650.011.605762544
CGCCGTA9850.011.369719547
AAAAAGG448950.010.98598670
GGGAAAA587050.010.76143159
AGCGTCG13700.010.72885811
GGGGAAA587400.010.4872558
AGATCGG16200.010.3825191
GGAAAAA692800.09.79048960
GGGGGAA710100.09.23700357
GATCGGA19100.09.1667742
GCGTCGT17200.08.74905312
AAAAAAG750700.08.728868569
CGTGTAG15450.08.38107816
AAGAGGG121000.08.360620570
GTGGTCG16550.08.24737542
GAAAAAA1007400.08.23728961
AGAGGGG152800.08.155539570
Produced by FastQC (version 0.11.5)