FastQCFastQC Report
Sat 23 Nov 2019
HWVCHAFXY_n02_WT-0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVCHAFXY_n02_WT-0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10520890
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5027154.778255451772616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG156400.09.60132170
AAAAGGG213950.09.0310370
AGGGGGG438150.08.2451531
GGGGAAA287300.08.077250559
GGGAAAA302300.07.861715359
GGGGGAA343250.07.46446358
AAGGGGG173550.07.44238670
AAAAAGG324950.07.17411270
GGAAAAA393650.06.757449660
AGAGGGG104450.06.434328670
CACACCC41600.06.0585434
CCCACAC40900.05.8184778
AAAAAAG536600.05.603310669
GAAAAAA635450.05.59602261
GGGAGAA118950.05.590731660
GGGGGGA722450.05.28098257
AGGGGGT38900.05.22445871
AAGAGGG91050.05.189963370
CCACACC53350.05.18377923
AGCGTCG13004.9762588E-55.114814310